Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2446816 | Journal of Experimental Animal Science | 2007 | 7 Pages |
Abstract
We aimed to evaluate the effects of intra-amniotic surfactant administration on alveolar lecithin/sphingomyelin ratio, density of type II pneumocytes, and fetal lung function in preterm merino sheep. Pregnant ewes at 119 days gestation either received 200Â mg intra-amniotic surfactant (n=4) or saline solution (n=4). After 24Â h, the lambs were delivered by hysterotomy and mechanically ventilated. Lecithin/sphingomyelin ratios in alveolar fluid, inflating pressure-volume relationships, and type II pneumocyte counts in histological specimens were compared among the groups. All of the lambs completed the protocol. Mean lecithin/sphingomyelin ratio increased significantly in amniotic (p=0.03) and alveolar fluid (p=0.03) samples of surfactant-treated animals. Lung function in terms of pressure-volume curves did not differ between two groups. Type II pneumocyte density tended to be higher (p=0.057) after intra-amniotic surfactant administration. Single-dose treatment with intra-amniotic surfactant seems to improve amniotic and alveolar lecithin/sphingomyelin ratio questionably by increasing alveolar type II cells. Pressure-volume relationships from inflation of the lungs might be unaltered with intra-amniotic surfactant treatment.
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Authors
M. Sezik, O. Ozkaya, E. Arslanoglu, A. Koker, H. Cetin, D. Ozbasar, H. Kaya,