| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2447314 | Livestock Science | 2014 | 5 Pages |
Egg yolk is most commonly used in semen extenders for cryopreservation but, wide variability in composition and potential risk of xenobiotic contamination has raised questions upon the use of egg yolk. Therefore, the present study was designed to develop a soya milk based phytoextender for buffalo semen cryopreservation. Soya milk was prepared from fresh soya beans in laboratory. Soya milk (25% v/v) and glycerol (6.4% v/v) was added in Tris citrate buffer to prepare Soya Milk Tris (SMT) extender. To improve post-thaw sperm quality, cryo-protectants like trehalose (100 mM) or taurine (50 mM) was supplemented in SMT extender before semen cryopreservation. Post-thaw quality parameters, degree of cryocapacitation and oxidative stress (lipid peroxidation) of spermatozoa cryopreserved in SMT with or without additives were assessed and compared with Egg Yolk Tris (EYT) extender. SMT extender showed no significant (P>0.05) differences in sperm motility, viability, membrane integrity and acrosome integrity as compared to EYT extender. The degree of cryocapacitation and oxidative damage were found significantly (P<0.05) lower in spermatozoa cryopreserved in SMT extender as compared to EYT extender. Supplementation of additives (trehalose or taurine) in SMT extender significantly (P<0.05) reduced oxidative damage. Thus, soya milk extender (25% soya milk v/v; 6.4% Glycerol in tris citrate buffer) with additives (trehalose or taurine) may substitute conventional egg yolk extender (20% egg yolk v/v; 7% Glycerol in tris citrate buffer) for better cryopreservation of buffalo semen.
