| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2448485 | Livestock Science | 2008 | 5 Pages |
Holstein heifers were randomly allotted by weight, age and body condition score to one of three treatments to test the hypothesis that GnRH administration concurrent with PGF2α injection would advance follicle or corpus luteum (CL) development parallel to an induced luteolysis of the pre-existing CL. Heifers in the control group (n = 14) received two treatments of PGF2α(25 mg, im) given 10 days apart. Groups 2 (n = 14) and 3 (n = 14) received an additional treatment of GnRH (100 μg, im) after the first and second PGF2α respectively. Estrus detection began immediately after PGF2α and continued for 80 h. Blood sampling was initiated 7 days prior to the first PGF2α (day − 7) and continued on days 0, 7, 10 (prior to the second PGF2α), 17 and 24. Heifers were artificially inseminated after the second PGF2α and pregnancy diagnosed at 60 days. There was a trend (P < .10) toward a lower estrus response in group 3 when compared to the other groups. Pregnant heifers in group 2 had lower progesterone (0.44 ± 0.09 vs. 1.72 ± 0.56 ng/ml) a week after the second PGF2α than the non-pregnant animals in that group (P < .05). Similar results were observed in the control group but only within the responding heifers (0.61 ± 0.08 vs. 0.93 ± 0.03 ng/ml; P < .05). Progesterone in heifers in group 2 remained high on day 0, 7, and 10 (1.48 ± 0.37, 1.23 ± 0.39, 1.96 ± 0.36 ng/ml) in spite of the treatment with PGF2α. This data suggest that administration of GnRH following PGF2α alters bovine luteal and/or follicular cell function.
