| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2454941 | Research in Veterinary Science | 2014 | 6 Pages |
•The roles of the PD-1/PD-Ls pathway during CSFV infection in swine are poorly understood.•The levels of PD-1 and its ligands mRNA were significantly increased in PBMCs during CSFV acute infection in swine.•Elevation of PD-1 and its ligands probably correlated with host immunosuppression during acute CSFV infection.•A novel mechanism of immunocompromised resulted by CSFV infection has been discovered.
In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection.
