Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2455172 | Research in Veterinary Science | 2012 | 5 Pages |
Abstract
Vaccination is an important measure to control Salmonella contamination in the meat production chain. A previous study showed that both the ÎrfaJ and ÎrfaL strains are suitable markers and allow serological differentiation of infected and vaccinated animals. The aim of this study was to verify whether deletion of the lon gene in a Salmonella Typhimurium ÎrfaJ marker strain resulted in decreased environmental survival. Our results indicate that deletion of the lon gene in the ÎrfaJ strain did not affect invasiveness in IPEC-J2 cells and resulted in an increased susceptibility to UV, disinfectants (such as hydrogen peroxide and tosylchloramide sodium) and citric acid. Immunization of pigs with inactivated ÎrfaJ or ÎlonÎrfaJ vaccines allowed differentiation of infected and vaccinated pigs. Furthermore, deletion of the lon gene did not reduce the protection conferred by live wild type or ÎrfaJ vaccines against subsequent challenge with a virulent Salmonella Typhimurium strain in BALB/c mice. Based on our results in mice, we conclude that deletion of lon in ÎrfaJ contributes to environmental safety of the ÎrfaJ DIVA strain.
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Authors
Bregje Leyman, Filip Boyen, Alexander Van Parys, Elin Verbrugghe, Freddy Haesebrouck, Frank Pasmans,