Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2455251 | Research in Veterinary Science | 2013 | 6 Pages |
To evaluate relative contributions of different microbial groups in rumen, the mono-culture (i.e. bacteria, protozoa and fungi) and co-cultures (i.e. bacterial–protozoal, fungal–protozoal and bacterial–fungal) were tested in vitro using high and low roughage diets. Total gas and methane were higher in bacterial–fungal and bacterial–protozoal co-cultures, while lower in fungal–protozoal than controls (high and low roughage with complete rumen consortia; control 1 and 2, respectively). Digestibility and total volatile fatty acids were lower in bacterial–fungal co-culture with both high and low roughage diets. Methanogens decreased in bacterial–fungal co-culture with high roughage. With high roughage, counts were lower for bacteria with bacterial–protozoal, protozoa with fungal–protozoal, and fungi with the bacterial–fungal co-cultures. Total gas was higher in bacterial mono-culture with low roughage, but methane was not detected in any mono-culture. Digestibility and total volatile fatty acids were significantly lowered with protozoal mono-culture. Methanogens reduced significantly in mono-cultures with high roughage diet than control 1. Defaunation reduced methanogens without significantly affecting rumen fermentation.