Trans-10, cis-12 conjugated linoleic acid directly enhances the chemotactic activity of porcine peripheral blood polymorphonuclear neutrophillic leukocytes by activating F-actin polymerization in vitro

Trans-10, cis-12 conjugated linoleic acid (t10c12-CLA) can reportedly alter the immune responses of phagocytes; however, it is unknown whether t10c12-CLA has a direct effect on the chemotaxis of peripheral blood polymorphonuclear neutrophillic leukocytes (PMNs). Here, we examined the effect of t10c12-CLA on the chemotaxis of porcine PMNs. The chemotactic response of porcine naïve PMNs was increased by porcine recombinant (pr) interleukin (IL)-8. Treatment with t10c12-CLA increased the chemotactic activity of porcine PMNs to IL-8 compared to porcine naïve PMNs, and enhanced their total cellular F-actin level. This increased chemotactic activity of t10c12-CLA-treated porcine PMNs was inhibited by cytochalasin D, an F-actin polymerization inhibitor. These results suggest that t10c12-CLA directly upregulates the chemotaxis of porcine PMNs, and that this effect may be associated with increased actin polymerization.