Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2456024 | Research in Veterinary Science | 2010 | 5 Pages |
The aim of the study was to evaluate the influence of a probiotic preparation on the genotoxicity of faecal water of broiler chickens fed with a fodder contaminated with aflatoxin B1 (AFB1) at 1 or 5 mg per kg. Human blood lymphocytes were exposed to chicken’s faecal water samples and DNA damage was measured using the comet assay. Genotoxicity of faecal water did not depend on the AFB1 concentration in the fodder. The mean DNA damage, measured as the percentage of DNA in the tail of the comets, for chickens fed with fodder with AFB1 at 1 mg/kg was 16.80 ± 0.66, at 5 mg/kg – 16.73 ± 1.51 and in the controls – 12.79 ± 0.66. The supplementation of fodder with the probiotic preparation decreased the extent of DNA damage to 10.02 ± 0.39 for 1 mg/kg AFB1 and to 11.89 ± 0.72 for 5 mg/kg.