Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2462022 | Veterinary Immunology and Immunopathology | 2011 | 6 Pages |
Abstract
Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
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Authors
Jella Wauters, Thierry Franck, Frederik Pille, Ann Martens, Kristel Demeyere, Stanislas Sys, Didier Serteyn, Frank Gasthuys, Evelyne Meyer,