| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2462119 | Veterinary Immunology and Immunopathology | 2011 | 7 Pages |
Abstract
Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.
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Authors
Saori Umeki, Ryoichi Suzuki, Masayuki Shimojima, Yasuo Ema, Takuma Yanase, Hiroyuki Iwata, Masaru Okuda, Takuya Mizuno,