| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2463385 | Veterinary Immunology and Immunopathology | 2006 | 10 Pages |
Abstract
We report the biochemical and functional properties of a novel bovine β-defensin (bBD-1). Cloned from bovine mammary papillary duct epithelia, the bBD-1 cDNA predicts a 69 amino acid propeptide that is much more similar to human β-defensin-1 (hBD-1) than to other bovine defensins. The bBD-1 gene contains two exons and one 8.5 kb intron. Using RT-PCR, we detected the bBD-1 transcript in the teat mucosa, kidney, vagina, ovary, oviduct, and colon. A synthetic bBD-1 peptide demonstrates potent antibacterial activity against Escherichia coli. The widespread expression of bBD-1 mRNA indicates that bBD-1 may play an important role in the bovine host defense against infections.
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Authors
Shelly Aono, Changqing Li, Guolong Zhang, Robert J. Kemppainen, Julie Gard, Wuyuan Lu, Xueyou Hu, Dean D. Schwartz, Edward E. Morrison, Christine Dykstra, Jishu Shi,