Article ID Journal Published Year Pages File Type
2463622 Veterinary Immunology and Immunopathology 2006 9 Pages PDF
Abstract

To gain further insight into the pathogenesis of porcine enzootic pneumonia (PEP), cytokine expression in different pulmonary compartments was examined. Mycoplasma hyopneumoniae (Mh) and proinflammatory and immunoregulatory cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-α) were detected by immunohistochemical methods in porcine lungs experimentally infected with Mh. Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 1- to 5-week post-inoculation (wpi). Three Mh-free pigs were taken as controls. Mh-antigen was shown in paraffin-wax-embedded tissues by immunohistochemistry in the luminal surface of bronchial and bronchiolar epithelial cells of all Mh-infected pigs. Significant increase in cytokine expression was detected on snap-frozen tissues from the bronchoalveolar exudate of the airways, mononuclear cells of the alveolar septa and macrophages and lymphocytes of the peribronchial and peribronchiolar lymphoid tissue, from 1 wpi onwards, compared to expression in non-pneumonic lungs. The main cytokines in the BALT of Mh-infected animals that showed an increase were IL-2, IL-4, IL-8, IL-10 and TNF-α. In the alveolar septa and bronchoalveolar exudate IL-1 (α and β), IL-2, IL-4, IL-8 and IL-10 expression also increased in infected animals.

Related Topics
Life Sciences Agricultural and Biological Sciences Animal Science and Zoology
Authors
, , , , ,