Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2466610 | Veterinary Microbiology | 2014 | 7 Pages |
Abstract
Zebrafish has been used for studying infections and host-pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2Â ÃÂ 107Â UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L. garvieae into macrophages as well as into non-phagocytic zebrafish cells of liver (hepatocytes). The internalization capacity showed by L. garvieae in zebrafish cells was confirmed in the rainbow trout cell line RTG-2. Our results provide the first evidence that L. garvieae is able to invade non-phagocytic host cells.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Mónica Aguado-Urda, Antonio RodrÃguez-Bertos, Ana I. de las Heras, MarÃa M. Blanco, Félix Acosta, Raquel Cid, José F. Fernández-Garayzábal, Alicia Gibello,