| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2470549 | Veterinary Parasitology | 2010 | 9 Pages |
The present study aims to evaluate the anthelmintic properties of aerial part of Macleaya microcarpa (Maxim) Fedde. Bioassay-guided fractionation and isolation of the compounds with anthelmintic activity were performed on the ethanolic extract of M. microcarpa yielding five bioactive alkaloids namely: sanguinarine, cryptopine, β-allocryptopine, protopine and 6-methoxyl-dihydro-chelerythrine by comparing spectral data (UV, NMR, and EI-MS) with literature values. According to in vivo anthelmintic assays, they were found to be 100% effective at the concentrations of 0.7, 8.0, 8.0, 16.0 and 7.0 mg l−1, and the median effective concentration (EC50) values for the five compounds were 0.37, 3.31, 4.64, 8.13 and 3.63 mg l−1, respectively. Additionally, the acute toxicity on goldfish for the five active compounds was also investigated with median lethal concentrations (LC50) values of 1.13, 16.12, 15.88, 21.69 and 10.91 mg l−1, respectively. The resulting therapeutic indices for sanguinarine, cryptopine, β-allocryptopine, protopine and 6-methoxyl-dihydro-chelerythrine were 3.03, 4.82, 3.40, 2.66 and 2.99 correspondingly. Correlations analysis between the log P and EC50, LC50 of the five alkaloids revealed that the activity of the five alkaloids was well correlated with their hydrophobicity and r2 = 0.45 is for anthelmintic activity while r2 = 0.47 is for acute toxicity for goldfish, respectively. These results provided evidence that the studied plant extract, as well as the isolated compounds, especially sanguinarine, might be potential plant-based medicines for the treatment of D. intermedius infection.
