Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2471694 | Veterinary Parasitology | 2008 | 4 Pages |
Interferon-gamma (IFN-γ) and interleukin-4 (IL-4) expression in crossbred (Bos taurus × Bos indicus) bovine calves during primary infection with Fasciola gigantica was measured. Ten crossbred calves of 1-year age were divided into two groups of five calves each, group I uninfected control and group II calves orally infected with a dose of 1000 metacercariae of F. gigantica. The two cytokines were measured 10, 30 and 75 days post-infection (PI) by real-time polymerase chain reaction (RT-PCR) with the double stranded DNA-binding dye SYBR Green. IL-4 was present in detectable levels in peripheral blood mononuclear cells (PBMCs) of infected animals at 10, 30 and 75 days PI but no IFN-γ was detected in PBMCs of infected animals at 10 and 30 days PI. However, at 75 days PI, IFN-γ in two infected animals was present in detectable level. Eosinophil count increased from 2nd fortnight after infection and the increased level persisted till the termination of experiment. Present study indicated that T-cell response during F. gigantica infection was Th2-type during earlier phase of infection, which may be polarized in chronic infection to that of a Th0-type.