Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25164 | Journal of Biotechnology | 2007 | 4 Pages |
Abstract
A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the specificity for 15β-hydroxylation was improved by mutating threonine residue 248 of CYP106A2 into valine. These new insights provide the basis for an optimized whole-cell steroid-hydroxylating biocatalyst that can be applied with an organic solvent phase.
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Authors
Harald J. Ruijssenaars, Eric M.G.M. Sperling, Peter H.G. Wiegerinck, Ferry T.L. Brands, Jan Wery, Jan A.M. de Bont,