Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2548847 | Journal of Pharmacological Sciences | 2015 | 5 Pages |
Abstract
Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (−)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.
Keywords
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Authors
Haruka Emori, Shinichi Iwai, Kakei Ryu, Hitoshi Amano, Takehiko Sambe, Takahiro Kobayashi, Tatsunori Oguchi, Kiyoshi Ohura, Katsuji Oguchi,