| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2823051 | Infection, Genetics and Evolution | 2013 | 6 Pages |
Orientia tsutsugamushi, an obligate intracellular bacterium closely related to the genus Rickettsia, is the causative agent of scrub typhus, a major cause of febrile illness in rural areas of Asia–Pacific region. Scrub typhus is transmitted by the bite of infected mites of the genus Leptotrombidium. The region of the 56-kDa TSA gene spanning from variable domain I (VDI) to variable domain IV (VDIV) was sequenced and used for genotyping 77 O. tsutsugamushi samples from human patients confirmed with scrub typhus from 2001 to 2003 and 2009 to 2010 in different regions of Thailand. These sequences were also compared to previously published 56-kDa TSA sequences. Only 4 genotypes out of 8 previously reported in Thailand were identified, i.e. Karp, JG-v, TA763 and Kato, respectively. Two strains were not associated with known genotypes but were closely related to Taiwanese strains. The Karp genotype was confirmed as the predominant clade. The JG-v and TA763 genotypes, in contrast to other studies, also were found. The genotype TA716 was not found, except for one strain previously described.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Only 4 genotypes out of 8 previously reported in Thailand were identified over 10 years. ► The main genotypes are Karp, JG-v, TA763 and Kato. ► Two strains were not associated with known genotypes but were closely related to Taiwanese strains. ► This is the first report of a spatio-temporal country-wide genotyping of Orientia tsutsugamushi.
