Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2823630 | Infection, Genetics and Evolution | 2007 | 5 Pages |
Abstract
This study has been carried out to get understanding of the origin among the strains of Mycobacterium leprae in patients from Northern India by using number of tandem repeats in rpoT gene as marker. Biopsies were collected from hundred leprosy cases (paucibacillary (PB) as well as multibacillary (MB)) across the spectrum from patients attending clinic at JALMA or diagnosed in Field Unit at Ghatampur (Kanpur). These biopsies were homogenized and DNA was extracted by a physiochemical procedure. rpoT region was amplified by using the primers and conditions earlier published. Among 100 strains from Northern Indian patients, 89% exhibited the presence of three copies of the 6Â bp tandem repeat in the rpoT gene, while 11% contained four copies. These profiles along with other genotyping data may help in studying the historical spread of leprosy by strains of M. leprae disseminated by various human races that migrated to Northern India from other places of Asian continent.
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Authors
Mallika Lavania, Kiran Katoch, Haribhan Singh, Ram Das, Anuj Kumar Gupta, Rahul Sharma, Devendra Singh Chauhan, Vishnu Dutt Sharma, Pawan Sachan, Shailendra Sachan, Vishwa Mohan Katoch,