Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2826125 | Trends in Plant Science | 2013 | 4 Pages |
Abstract
Fluorescent protein (FP) fusions are frequently used to localize and follow the movement of proteins in living cells. However, a consensus is missing about the experimental design and controls that guarantee the reliability of the results. Here, we discuss possible artifacts and try to navigate through the many methods, preferences, and assumptions that surround protein localization in plants that make it difficult to design a universal approach to achieve reliable results.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Francesca M. Quattrocchio, Cornelis Spelt, Ronald Koes,