Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2836196 | Physiological and Molecular Plant Pathology | 2016 | 9 Pages |
•Novel type I RIPs were detected in oil palm.•EgRIPs expression levels were altered during plant-pathogen interaction.•EgRIPs demonstrated depurination activity.•EgRIPs inhibited Ganoderma boninense mycelial growth in vitro.
Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28–30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense.