| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2836248 | Physiological and Molecular Plant Pathology | 2015 | 13 Pages |
•Differentially-regulated genes due to C. musae infection in banana peel were identified.•Most up-regulated defence-related genes were homologous to germin-like proteins.•Anthracnose lesion development (LD) and fruit ripening (FR) were positively correlated.•Increased expression of GGT, GLP and β-1,3-glucanase correlated positively with LD and FR.•Relative expression of GGT had a positive correlation with that of β-1,3-glucanase.
The expression profiles of genes in banana cultivar (Seenikesel) due to infection of Colletotrichum musae was analysed and functions of differentially-regulated expressed sequence tags (ESTs) were deduced. Among the up-regulated ESTs, 51%, 16% and 11% were related to defence response-, stress response- and energy and metabolism-related genes, respectively. Moreover, 22% and 11% of the down-regulated genes, respectively accounted for defence and stress response-related functions and 11% for protein synthesis, folding and stabilization. In addition, similarly-regulated gene transcripts were identified and 30%, 30% and 20% of them were respectively related to defence, energy and metabolism and protein synthesis folding and stabilization functions. Relative expression of three up-regulated gene transcripts, namely gamma glutamyl transferase (GGT), germin-like protein (GLP) and β-1,3-glucanase on peel tissues over a period after inoculation of C. musae was analysed. Relative fold increase of expression of the three genes showed a positive correlation with anthracnose lesion development and ripening. This study elucidated the genetic background of resistance of cultivar Seenikesel, possible mechanisms of resistance and correlated the expression of selected gene transcripts towards C. musae infection and fruit ripening.
