| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2836329 | Physiological and Molecular Plant Pathology | 2012 | 7 Pages |
The biotrophic mycoparasite-host interface and replacement remain poorly described, yet their understanding is important for effective biocontrol of Fusarium plant pathogens. Alcohol percentage test (APT) and real-time PCR quantification of DNA were employed to evaluate the efficiency of mycopathogenic Sphaerodes mycoparasitica mycelia to replace an array of pathogenic/toxigenic Fusaria in co-cultures. Shifts in Fusarium mycelial growth and hydrophobicity measured (APT) at the interaction zone with S. mycoparasitica indicated the degree of Fusarium mycelia replacement by the mycoparasite (P < 0.05); while qRT-PCR accurately detected DNA quantity changes in mycotoxigenic Fusarium graminearum 3-acetyldeoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON) chemotypes challenged with S. mycoparasitica. Results from the HPLC experiments also demonstrated a considerable decrease in mycotoxin (DON, 3-ADON, 15-ADON, and ZEA) concentrations at the contact zone. Estimated shifts in the DNA quantity and mycotoxin production, assessed using qRT-PCR and HPLC respectively, correlated with an alteration in APT values, demonstrating the effectiveness of the mycoparasite in vivo.
Graphical abstractThe original data presented in this paper shed light on shifts in hydrophobic properties and DNA quantities of pathogenic and mycotoxigenic F. avenaceum, F. oxysporum, F. graminearum 3-Acetyldeoxynivalenol (3-ADON) and 15-Acetyldeoxynivalenol (15-ADON) chemotypes challenged with biotrophic – Fusarium-specific – mycoparasite.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Sphaerodes mycoparasite against pathogenic and mycotoxigenic Fusaria. ► Biotrophic mycoparasite-Fusarium graminearum chemotypes interactions. ► qRT-PCR to assess the amount of fungal DNAs. ► Alcohol percentage test (APT) to assess the level of hydrophobicity. ► HPLC to assess the mycotoxin production performance.
