Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2836330 | Physiological and Molecular Plant Pathology | 2012 | 6 Pages |
Development of aflatoxin-resistant cotton against Aspergillus flavus is handicapped by the lack of resistance source in available germplasm. Genetic engineering warrants for identification of resistance-associated genes in cotton. As a first step toward this, we isolated 44 differentially expressed genes (DEGs) in response to A. flavus infection, using annealing control primer system. Different functional classification of the DEGs suggested a complex and multi-factorial plant–fungus interaction. Eight DEGs, including transcription factors, kinase, and downstream stress responsive genes, showed a tissue- and time-dependent differences in their expression. The upregulated genes can be used as transgenes and/or functional markers for breeding aflatoxin-resistant cottonseed.
► Cotton genes involved in the primary stage of Aspergillus flavus infection. ► Transcription factors and protein kinases as key candidates. ► Annealing control primer system to identify differentially expressed genes in cotton. ► Cross-talk between A. flavus infection and other abiotic stresses.