Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2836530 | Physiological and Molecular Plant Pathology | 2011 | 9 Pages |
We determined the temporal and spatial localization of the phytoalexin avenanthramide A, and its biosynthetic enzyme, hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxy-cinnamoyltransferase (AsHHT) in oat leaves infected with the crown rust fungus. Accumulation of avenanthramide A and AsHHT was first observed predominantly in hypersensitive response (HR) cells at 36 h post inoculation (hpi), and later in adjacent cells at 48 hpi. At 120 hpi, avenanthramide A was detected in a wider area of infected tissues, but AsHHT-positive signals were only observed in the HR and adjacent cells, suggesting that avenanthramide A was synthesized around the HR cells, and then transported to other parts of the infected tissues in a center-to-periphery manner.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The phytoalexin, avenanthramide A was first synthesized at the sites of infection. ► Avenanthramide A was then transported to other parts in the infected tissues. ► PR-10 expression was also induced upon fungal infection at the very early stage. ► Further upregulation of PR-10 occurred only in the incompatible interaction.