Functional characterization of an aquaporin in the Antarctic midge Belgica antarctica

Aquaporins (AQPs) are water channel proteins facilitating movement of water across the cell membrane. Recent insect studies clearly demonstrate that AQPs are indispensable for cellular water management under normal conditions as well as under stress conditions including dehydration and cold. In the present study we cloned an AQP cDNA from the Antarctic midge Belgica antarctica (Diptera, Chironomidae) and investigated water transport activity of the AQP protein and transcriptional regulation of the gene in response to dehydration and rehydration. The nucleotide sequence and deduced amino acid sequence of the cDNA showed high similarity to AQPs in other insects and also showed characteristic features of orthodox AQPs. Phylogenetic analysis revealed that Belgica AQP is a homolog of dehydration-inducible AQP of another chironomid, Polypedilum vanderplanki. A swelling assay using a Xenopus oocyte expression system verified that Belgica AQP is capable of transporting water, but not glycerol or urea. The AQP mRNA was detected in various organs under non-stressed conditions, suggesting that this AQP plays a fundamental role in cell physiology. In contrast to our expectation, AQP transcriptional expression was not affected by either dehydration or rehydration.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We cloned an aquaporin cDNA from the Antarctic midge Belgica antarctica. ► This AQP is a homolog of a dehydration-inducible AQP found in another chironomid. ► The Belgica AQP is capable of transporting water, but not glycerol or urea. ► The AQP mRNA is expressed in diverse organs under non-stressed conditions. ► This AQP is not transcriptionally regulated by either dehydration or rehydration.