High-throughput automation design considerations for biotechnology processes involving RNA purification protocols using multi-centrifuge bioseparation steps

Design of an automation line is a multi-objective optimization problem involving throughput, yield, floor space and cost constraints. The paper examines the feasibility of a computer-aided automation design for biotechnology applications using Arena™ software. A generic case study chosen for this study involves a sequence of steps in a preparation process of RNA from tissue-cultured cells. These steps involve repetitive usage of centrifuging operations to perform separation of biochemical substances. A sample must be loaded onto a centrifuge by a pick-and-place device (typically a robot manipulator). Consecutive centrifuging steps may involve multiple centrifuges as well as robots, or some measure of equipment sharing. The paper proposes a unified simplified cost model for all design objectives (throughput, space utilization, process capital and operational cost) and a quantitative selection criterion to allow for an optimal automation design.