Plant growth-promoting traits of yeasts isolated from the phyllosphere and rhizosphere of Drosera spatulata Lab.

•Yeasts from the Drosera spatulata Lab. were isolated and identified.•All yeast isolates were tested for plant growth-promoting traits.•The antagonistic action of these yeasts against fungal pathogen was evaluated.•The cocultivation of tobacco with these yeasts enhanced plant growth.•We provides an eco-friendly approach for sustainable agriculture development.

Microorganisms can promote plant growth through direct and indirect mechanisms. Compared with the use of bacteria and mycorrhizal fungi, the use of yeasts as plant growth-promoting (PGP) agents has not been extensively investigated. In this study, yeast isolates from the phyllosphere and rhizosphere of the medicinally important plant Drosera spatulata Lab. were assessed for their PGP traits. All isolates were tested for indole-3-acetic acid-, ammonia-, and polyamine-producing abilities, calcium phosphate and zinc oxide solubilizing ability, and catalase activity. Furthermore, the activities of siderophore, 1-aminocyclopropane-1-carboxylate deaminase, and fungal cell wall-degrading enzymes were assessed. The antagonistic action of yeasts against pathogenic Glomerella cingulata was evaluated. The cocultivation of Nicotiana benthamiana with yeast isolates enhanced plant growth, indicating a potential yeast–plant interaction. Our study results highlight the potential use of yeasts as plant biofertilizers under controlled and field conditions.