Agni’s fungi: heat-resistant spores from the Western Ghats, southern India

This study concerns the thermotolerance of spores of mesophilic fungi isolated from a tropical semi-arid habitat subject to dry season fire in the Western Ghats, southern India. Among 25 species of Ascomycota isolated from leaf litter, nine were able to grow after incubation in a drying oven for 2 h at 100 °C; the spores of two of these species survived 2 h incubation at 110 °C, and one survived exposure to 115 °C for 2 h. The range of thermotolerance among mesophilic fungi isolated from the leaf litter was surprising: filamentous fungi from other habitats, including species that colonize scorched vegetation after fires and thermophilic forms occurring in self-heating plant composts, cannot survive even brief exposure to such high temperatures. It is possible that the exceptional heat resistance of the Indian fungi is related to adaptations to surviving fires. Genetic analysis of the physiological mechanisms of heat resistance in these fungi offers prospects for future biotechnological innovations. The discovery of extreme thermotolerance among common saprotrophs shows that this physiological trait may be more widespread than recognized previously, adding to concern about the evolution of opportunistic pathogens on a warmer planet. The fungi in this study are among the most heat-resistant eukaryotes on record and are referred to here as ‘Agni’s Fungi’, after the Hindu God of Fire.

► We report remarkable heat resistance of fungi isolated from a semi-arid habitat subject to fires. ► Spores survive rapid heating above the boiling point of water. ► These microbes are among the most heat-resistant eukaryotes. ► This study raises concern about the evolution of opportunistic fungal pathogens on a warmer planet.