Evaluation of partial tef1, rpb2, and nLSU sequences for identification of isolates representing Armillaria calvescens and Armillaria gallica from northeastern North America

► Partial tef1 sequences were able to discriminate between 24 isolates (12 each) of A. calvescens and A. gallica. This was determined from a set of isolates that originated from across northeastern North America using both maximum likelihood and maximum parsimony methods. ► In addition to A. calvescens and A. gallica, partial tef1 sequences were also able to distinguish between isolates representing the remaining four northeastern Armillaria species (A. gemina, A. mellea, A. sinapina, and A. solidipes). ► Partial rpb2 and nLSU sequences were not able to distinguish isolates representing A. calvescens and A. gallica in this study.