Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4374662 | Ecological Indicators | 2007 | 13 Pages |
Abstract
The individual cytotoxicity of cadmium chloride, iron sulphate and chromium nitrate has been investigated by using the freshwater ciliate Tetrahymena pyriformis. The metabolic enzymes and antioxidant defense biomarkers were assessed. The results obtained reveal that their metal salts have perturbed the physiology and morphology of T. pyriformis. Also, the biomarkers assessed were sensitive to the presence of metal salts and this sensitivity was metal salt and dose dependant. To estimate the impact of their metal salts on mitochondria, we studied their effects in vivo and in vitro on the d-β-hydroxybutyrate dehydrogenase (BDH) (EC 1.1.1.30) inner mitochondrial membrane enzyme. The results showed a high inhibition of BDH in terms of activity, protein expression and kinetic parameters.
Keywords
MDAd-glyceraldehyde-3-phosphateDTTNADPHBDHdithionitrobenzeneDTNBTetrahymena pyriformisPMSFDCIPdithiotreitolKCNNAD(H)Metabolic enzymesEDTAethylenediamine tetraacetic acidAntioxidant biomarkersdichloroindophenolCytotoxicityPhenylmethylsulphonylfluoridemalondialdehydeMitochondriaMetal saltsnicotinamide adenine dinucleotide phosphate reduced formpotassium cyanide
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Authors
Driss Mountassif, Mostafa Kabine, Rachid Manar, Noureddine Bourhim, Zaina Zaroual, Norbert Latruffe, M'Hammed Saïd El Kebbaj,