Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4381576 | Acta Oecologica | 2008 | 8 Pages |
Abstract
Inorganic carbon removal through acidification is a common practice prior to isotopic analysis of macroinvertebrate samples. We have experimentally tested the effect of acidification on the elemental and isotopic composition of a range of beach arthropod species. Acidification resulted in a significant depletion of 7.7% and 1.2% on average for carbon and nitrogen, respectively, suggesting that acid washing affects body carbon compounds other than carbonates. With a few exceptions, δ13C and δ15N showed no changes following 1 N HCl attack. Based on those exceptions, our results show that only those samples with a high CaCO3 content result in impoverished 13C as a consequence of acidification. Those suspected to be carbonate-free are not significantly affected. Concerning δ15N values, only high carbonate species were affected when treated with HCl. As a standard protocol, it is recommended to acidify only carbonate-rich samples prior to δ13C analyses. When possible, muscle tissue samples should be used instead of the entire organism.
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Authors
Oscar Serrano, Laura Serrano, Miguel Angel Mateo, Isabella Colombini, Lorenzo Chelazzi, Elena Gagnarli, Mario Fallaci,