| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4382096 | Applied Soil Ecology | 2015 | 6 Pages |
•We analyze the polymorphism of chlorpyrifos-degrading strains in soil.•The residue periods of chlorpyrifos in soil under microcapsules is prolonged.•Chlorpyrifos microcapsule can enhance the degrading ability of soil microbes to it.•Improper use of microencapsulated pesticide may shorten its service life.
The molecular polymorphisms of chlorpyrifos (CPF)-degrading strains in soil treated with microcapsule (MC) and emulsifiable concentrate (EC) formulations of CPF were studied by laboratory experiments. Results showed that CPF residue in MC-treated soil was maintained at a certain level during 60 days, and the residual period reached 120 days. CPF residue decreased via a first-order model with a residual period of 60 days in EC-treated soil. A total of 16 and 9 degrading strains capable of utilizing CPF as a sole source of carbon and energy were isolated from these two treatments, respectively. All degrading strains were grouped into 16 different types based on enterobacterial repetitive intergenic consensus (ERIC)-PCR pattern similarities. Strains isolated from MC- and EC-treated soils belonged to 14 and 8 ERIC-types, respectively. Six groups of strains that were isolated from MC- and EC-treated soils belonged to similar ERIC-types. All ERIC-type strains were similar to nine known species according to BLAST analysis at the NCBI website. The results indicated that the population and degradation abilities of CPF-degrading strains in soil were visibly strengthened by the controlled-release formulation of CPF compared with those by the non-controlled release formulation.
