Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4382842 | Applied Soil Ecology | 2011 | 8 Pages |
One of the successful, safe and effective methods to introduce bioinoculants in soil is encapsulation of cells in biodegradable gel matrices which not only releases the microorganisms to the soil gradually but also helps to increase the survival rate by protecting them against many environmental stresses. Two bacterial strains viz. Pseudomonas fluorescens BAM-4 and Burkholderia cepacia BAM-12 were immobilized using sodium alginate and alginate + skim milk as carrier to check the phosphate solubilization in vitro and were found to have significantly higher activity than control. Their efficacy was also tested in vivo on wheat plants growth in semi arid environmental conditions in pots. The seed inoculation of rhizobacteria along with hardly soluble TCP and RP significantly (P < 0.05) enhanced the overall development of wheat plants in free and immobilized cell states in comparison to control. With a few scattered exceptions, plants amended with bacterial strains showed significantly (P < 0.05) better performance than the plants having chemical fertilizer, SSP and biofertilizer, biogold. When the two insoluble P sources were compared, TCP was found much better than RP treatments. Performance wise, B. cepacia BAM-12 was found to be the best.
► Pseudomonas fluorescens BAM-4 and Burkholderia cepacia BAM-12 were immobilized using sodium alginate and alginate + skim milk as carrier to check the phosphate solubilization in vitro and were found to have significantly higher activity than control. ► The seed inoculation of rhizobacteria significantly (P < 0.05) enhanced the overall development of wheat plants. ► When two insoluble P sources were compared, TCP was found much better than RP treatments. ► Performance wise, B. cepacia BAM-12 was found to be the best.