The genotoxic effect of actinomycin D on micronuclei of bone marrow cells and serum protein electrophoretic pattern of mice

The genotoxic effects of actinomycin D (ActD) were assayed in an in vivo test utilizing bone marrow cells and serum protein electrophoresis assay. ActD (0.0005%) was dissolved in 0.9% saline and administered intraperitoneally at a volume of 1 ml solution per 100 g body weight. Five groups of male mice, were intraperitoneally injected with ActD and were sacrificed after 2, 6, 24, 36 and 48 h. The number of the micronucleated cells increased gradually with increasing sampling time from 2 to 36 h. At 48 h the micronuclei frequencies in bone marrow cells of the treated animals were still elevated when compared to the MN frequency in the control mice. No significant difference in the polychromatic erythrocytes (PCEs) percentage, normochromatic erythrocytes (NCEs) percentage and PCEs/NCEs ratio was found in all the treated groups compared to the control. The electrophoretic patterns of serum protein showed great changes in shape, number and intensities of the protein bands.