Simultaneous Determination of Hexoestrol, Diethylstilbestrol, Estrone and 17-Beta-estradiol in Feed by Gas Chromatography-mass Spectrometry

A method was developed for the simultaneous determination of four kinds of estrogens (hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol) in feed by gas chromatography-mass spectrometry (GC-MS). After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatography-mass spectrometry. The results showed that the linear detectable ranged from 2.5 ng · mL−1 to 250 ng · mL−1 for hexoestrol and from 5 ng · mL−1 to 500 ng · mL−1 for three other estrogens with the correlation coefficients (R2) were no less than 0.990. The recoveries were in the range of 76.34%–96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation (LOQ) for all analytics were between 10 ug · kg−1 and 20 ug · kg−1. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.