Clone and Bioinformatics Analysis of Pig SRPK1 Gene 5′ UTR Region

Part 5′ UTR region of pig SRPK1 gene was cloned by inverse PCR (I-PCR), then a 425 bp gene sequence was acquired. A promoter region in −1-−309 bp was predicted by online tool (TFSEARCH) and 32 binding sites of transcription with scores higher than 85 were getten, of which scores of Sp1, MyoD, and HSF2 were over 90. Some of these binding sites of transcription factors were connected with promoters, but TATA-box, which was important to gene expression, hadn't been found in this region. By using PCR-SSCP method to search SNPs this part (5′ UTR of SRPK1 in pig), total of 40 Large White pigs were obtained as the research objects, but no polymorphism were found. Thus, 5′ UTR of SRPK1 was speculated with a characteristic of high conservation, while it might have been directly or indirectly selected in commercial breeding. The paper provided a further feature of SRPK1 gene in molecular genetics.