In vitro transcriptomic prediction of hepatotoxicity for early drug discovery

► In vitro-derived models are effective in predicting drug hepatotoxicity on in vivo animals and in vitro liver cells. ► Our prediction model accurately stratified rat and human liver cells treated with various compounds of liver toxicity. ► Introducing a novel methodology that can be readily generalized to develop assays for many other types of drug toxicity. ► Accelerating the “personalized toxicogenomics” to tailor drug treatments based on patients' toxicity profiles.