| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4503211 | Acta Agronomica Sinica | 2011 | 10 Pages |
A molecular marker-assisted selection (MAS) strategy was used to improve resistance to Rice stripe virus (RSV) in rice (Oryza sativa L. subsp. japonica cv. Wuyunjing 8). Rice variety “Aoikaze” with resistance to RSV was used as the donor. Sequence-tagged site (STS) markers STS11-31, STS11-7, STS11-19, and STS11-43 anchoring the resistance loci were used for MAS in the backcross progenies. In 2008, 70 lines of BC3F5 and 115 lines of BC4F4 population were obtained with stable agronomic traits after MAS assay. Further evaluation of agronomic traits, yield, grain quality, and resistance to RSV were carried out in these backcross progenies, and 10 BC4F5 lines and 2 BC3F6 lines showed similar phenotypes to Wuyunjing 8. The 12 lines were finally selected for the improved resistance to RSV and the high-yield and elite-quality of Wuyunjing 8.
摘要采用农杆菌介导法将红色荧光蛋白基因DsRed转入轮枝镰孢Fv-1菌株, 利用荧光显微镜观察轮枝镰孢在玉米自交系B73根部定殖和生长的规律。土壤中的轮枝镰孢首先侵染玉米的须根等组织, 并在其中大量增殖, 随后沿主根向上侵染, 以菌丝的形式扩展到地上组织。有些孢子附着在根表面的纹理中, 萌发形成菌丝而扩展; 有的则向内侵染附着的细胞, 然后再继续向周边侵染。由根内部向上侵染的菌丝多沿着细胞间隙上行, 有些也会穿行在不同细胞之间。分析接种不同时间轮枝镰孢在玉米根和茎基部组织形成的单菌落数量(CFU)发现, 轮枝镰孢在根部的CFU值随时间逐渐减小, 而茎基部的CFU值则呈逐渐增大的趋势。这说明土壤中的轮枝镰孢能够通过根系侵染途径危害地上部组织。本研究的结果为进一步探明轮枝镰孢和玉米之间的互作关系, 以及其他土传真菌与植物之间的互作提供了有益的参考。
