| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4503260 | Acta Agronomica Sinica | 2010 | 7 Pages |
Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (Manihot esculenta Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.
摘 要利用SRAP标记, 选用36对多态性较好的引物组合, 对18个木薯品种进行分析鉴定, 扩增出320个位点, 其中多态性位点235个, 多态性比率达73.4%, 平均每对引物组合可产生8.9个位点和6.5个多态性位点; 235个多态性位点采用非加权组平均法(UPGMA)进行聚类分析, 以遗传相似系数0.734为阈值, 可将18份供试材料分为4组; 选用2对多态性引物Me1-Em5和Me24-Em10, 初步构建了18份木薯品种的指纹图谱, 根据条带的有无转换为0、1二进制编码形成数字指纹, 每个品种拥有唯一的数字指纹区别于其他品种, 置信概率达到99.999%。结果表明, 采用SRAP标记建立的指纹图谱适用于木薯品种的分类和鉴定。
