| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4503312 | Acta Agronomica Sinica | 2010 | 9 Pages |
The purpose of this study was to provide simple sequence repeat (SSR)-based DNA fingerprinting information for sugarcane (Saccharum spp. Hybrids) cultivars that were developed in Yunan Province, China. Genomic DNAs from 27 cultivars were amplified with 8 pairs of polymorphic SSR primers. A total of 129 bands were obtained, including 123 polymorphic bands (95.35%). The mean value of polymorphic information content (PIC) was 0.9445, and the genetic similarity coefficient ranged from 0.269 to 0.767. Markers SMC1047HA and MSSCIR21 had the highest PIC values of 0.963 and could distinguish all the cultivars tested. Besides, primer combinations MSSCIR36/MSSCIR21, MSSCIR16/MSSCIR36, and MSSCIR36/SMC336BS were efficient in identifying the 27 cultivars, and their similar coefficients were lower than those of other primer combinations. These 3 primer combinations were validated with 27 cultivars and 10 staple commercial sugarcane cultivars in China, and MSSCIR16/MSSCIR36 was found to be optimal to construct DNA fingerprint identity (ID). Nomenclature of DNA fingerprint ID was suggested as serial number of National Nursery of Sugarcane Germplasm Resources (NNSGR) plus country and region code plus English initial abbreviation of breeding institution plus core primer name plus SSR marker data. This system is informative and useful in protecting new varieties and identifying cultivars because it consists of not only important information of a cultivar but also its specific molecular identify.
摘 要以云南27份甘蔗自育品种为材料, 从国际微卫星协会提供的120对SSR引物中筛选出8对多态性丰富、品种聚类区分率高、易统计的引物组成核心引物。8对SSR引物共产生129条带, 123个为多态带, 多态条带比例为95.35%, 多态信息量平均为0.9445, 品种相似性系数在0.269∼0.767之间, 其中引物SMC1047HA, MSSCIR21不仅多态性丰富, 而且单个引物就可区分所有品种, 是最有效的核心引物。8对核心引物两两组合的效率分析表明, MSSCIR36/MSSCIR2、MSSCIR16/MSSCIR36和MSSCIR36/SMC336BS是高效引物组合, 可以完全有效区分所有品种, 且品种相似性系数较低; 同时使用蔗区种植面积较大的10个主栽品种验证3个高效引物组合, 结果表明, MSSCIR16/MSSCIR36是最佳引物组合, 不仅能有效区分所有云南甘蔗自育品种, 而且能将云南甘蔗自育品种与10个主栽品种最有效地区分开。使用品种的国圃号、国家地区代码、育种单位英文缩写、核心引物名称和分子数据组成云南甘蔗自育品种的DNA指纹身份证, 不仅包含了品种的重要信息, 而且其中的分子数据可用于品种的真伪鉴定和遗传关系分析, 为品种的知识产权保护提供有效的科学依据。
