QTL Analysis for Dynamic Expression of Chlorophyll Content in Soybean (Glycine max L. Merr.)

The objective of this study was to map quantitative trait loci (QTLs) for chlorophyll content in the F2:3 and F2:4 populations derived from soybean (Glycine max L. Merr.) varieties Lishuizhongzihuangdou and Nannong 493-1. Chlorophyll contents for the function leaf in vivo were measured from seedling to blooming stage in the 244 F2:3 and F2:4 families growing in 2 environments. The developmental behaviors of chlorophyll content across environments were analyzed using composite interval mapping in the software of Windows QTL Cartographer v2.5. A total of 20 QTLs were detected at various stages in 2 environments, of which only several QTLs were common across stages, years, or test sites. QTLs distributing in linkage groups N, D1a, F, and K were repeatedly detected for 3 to 4 times. QTL qchl-D1a-1, which was located on linkage group D1a between markers sat_160 and satt147, was only identified at Jiangpu site in both years. Three common QTLs were mapped on linkage groups K, M, and N at both Jiangpu and Linyi sites. These results provide a theoretical basis for genetic analysis of chlorophyll traits and marker-assisted breeding in soybean.

摘 要采用溧水中子黄豆×南农493-1后代衍生的244个F2单株及筛选的150个SSR分子标记构建的连锁遗传图谱, 在苗期至开花期测定F2衍生F2:3和F2:4家系生长正常单株的倒3复叶功能叶(非离体)的叶绿素含量13次, 通过Windows QTL Cartographer v2.5软件包的复合区间法, 动态定位了大豆叶绿素含量的QTL。结果表明, 不同时间点共检测到20个QTL, 其中, 不同发育阶段间、年份间和地点间共同的QTL较少, 不同时间点上的QTL差异较大, 重复出现在N、D1a、F和K连锁群的QTL有3∼4次。这些结果为叶绿素含量的遗传剖析和标记辅助育种提供理论依据。