Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4503413 | Acta Agronomica Sinica | 2009 | 6 Pages |
The objective of this study was to survey and identify proteins in soybean (Glycine max) cultivar Suinong 10 in response to inoculation of Phytophthora sojae by means of 2-dimensional gel electrophoresis and mass spectrometry techniques. In the 19 differentially expressed products obtained, 12 proteins were up-regulated and 6 proteins were down-regulated. The remaining one protein was specifically expressed after inoculation. Ten protein spots were assayed with biological mass spectrometry, including 1 specifically expressed, 1 down-regulated, and 8 up-regulated spots. These 10 proteins were involved in 8 functional proteins that were categorized into 4 groups: the ribolose-1,5-phosphate carboxylase large subunit and its precursor and succinyl coenzyme A comprising the metabolism group; kinase receptor like protein, oxidoreductase, and cysteamine ammonia acid oxidoreductase constituting the group of transport of metabolites in cells; the clothing zeta-3 protein subunits falling in the signal transduction group; and the F-box protein involved in jasmonic acid-mediated resistance. These differentially expressed proteins may be responsible for resistance of Suinong 10 to P. sojae and require further verifications.