Obtaining Intergeneric Hybrids Between Brassica Species (B. juncea and B. nigra) and Orychophragmus violaceus via Ovary Culture

Intergeneric hybridization is an effective way to create new germplasm as breeding materials. Reciprocal crosses between two Brassica species, B. juncea and B. nigra, and Orychophragmus violaceus were carried out to transfer desirable genes from O. violaceus to B. juncea. The ovaries of hybrids were cultured on 6 media. The intergeneric crossability showed obvious differences among crosses, media, Meanwhile, the intergeneric crossability was also affected by female parents. Nine out of 10 hybrid plants possess morphological similarity to the female parent B. juncea or the intermediate between 2 parents. In the hybrid derived from the cross between Younensi, a variety of B. nigra, and O. violaceus, one plant was completely male sterile, and showed variations in branching pattern and leaf morphology. The 10 hybrid plants were validated to be real intergeneric hybrids through morphological identification and analysis of microsatellite markers.