Enhancing bioefficacy of Bacillus subtilis with sodium bicarbonate for the control of ring rot in pear during storage

Pear ring rot caused by Botryosphaeria berengeriana is a common disease throughout pear-growing areas. The use of biocontrol agents to control the disease can potentially reduce the negative effects of chemical fungicides on fruit and on the environment. Here, we evaluated the preventative (before pathogen inoculation) and curative (after pathogen inoculation) effects of biocontrol agents (BA) with sodium bicarbonate (SBC) against B. berengeriana alone or in combination on plates and on fruit. The order of treatments applied in the combinations, and two different doses were also evaluated. The inhibition rates were 42.30%, 75.77–85.41%, and 83.00–90.23% in vitro with 2% SBC (w/v), BA (strains 11, 28, 2 and sf 628), and combination treatments, respectively. On fruit, the curative inhibition rates were 23.83%, 12.79%, 71.09%, 48.16%, 74.57%, and 57.90% when treated with BA 11 (30 mL), SBC (30 mL), BA 11 (15 mL) + SBC (15 mL), SBC (15 mL) + BA 11 (15 mL), BA 11 (30 mL) + SBC (30 mL), and SBC (30 mL) + BA 11 (30 mL), respectively; the preventative inhibition rates were 28.72%, 17.31%, 78.25%, 51.06%, 80.91%, and 61.50%, respectively. Combinations were superior to individual treatments and significantly better with SBC following BA than vice versa. Therefore, the proper combination of SBC with BA can be developed as a promising approach against pear ring rot during storage.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► Bioefficacy of Bacillus subtilis with NaHCO3 against B. berengeriana was evaluated. ► The combinations were superior to individual treatments in vitro and in vivo. ► It’s significantly better with NaHCO3 following Bacillus subtilis than vice versa. ► It can be developed as a promising approach against pear ring rot during storage.