Dissemination of entomopathogenic fungi using Busseola fusca male as vector

The stem borer, Busseola fusca (Fuller), is an important pest of maize Zea mays L. and sorghum Sorghum bicolor (L.) in eastern and southern Africa. To control this pest, biological control methods including the use of entomopathogenic fungi are being considered. The pathogenicity of one isolate of Metarhizium anisopliae (Metsch.) Sorok. and one isolate of Beauveria bassiana Bals. (Vuill.) were first tested on different developmental stages of B. fusca including eggs, neonate, 2nd and 3rd-instar larvae. Both fungal isolates were pathogenic to all the stages tested. However, differences in mortality were observed among larvae that hatched from treated egg masses. Experiments were conducted thereafter to test whether B. fusca males could serve as a vector for fungal conidia to contaminate B. fusca females and subsequently eggs and larvae. Results demonstrated that B. fusca males successfully transferred inoculum to females during copulation, which in turn transmitted it to the eggs they laid on maize plants, resulting in the decrease of leaf damages.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We tested the pathogenicity of two fungal isolates on different developmental stages of Busseola fusca, one of the main pest of maize in Sub-Saharan Africa. ► We tested whether B. fusca males could serve as vector for fungal conidia to contaminate B. fusca females and subsequently eggs and larvae. ► Both fungal isolates were pathogenic to all the stages tested. ► B. fusca males successfully transferred inoculum to females and eggs, resulting in the decrease of maize-leaf damages.