Effects of wetting on the persistence of quiescent conidia of Isaria fumosorosea

Quiescent conidia of Isaria fumosorosea were submitted to various wetting–drying cycles under different regimes of temperature and air humidity. Germination and viability of conidia collected on cultures freshly host-passed (P2) were not affected at 25 °C during five cycles at increasing wet phase duration (2–12 h per daily cycle) under any moisture conditions (13–86% RH). Infectivity levels remained stable, but mortality was slightly postponed. In vitro-cultured inocula (P5) were significantly affected after only one cycle at higher air humidity (75 and 86% RH) and temperature (35 and 40 °C). The persistence of I. fumosorosea conidia suspended in water soluble extracts of leaf surfaces (corn and cabbage) confirmed the better persistence of P2 conidia and the relatively higher detrimental effect of lower air humidity conditions when combined with moderate temperatures. Quiescent conidia deposited in situ on potted plants of cabbage showed a higher persistence on wet foliage and on foliage submitted to wetting–drying cycles, than on dry foliage. These results underline that constraints prevailing in targeted environments and ecological fitness of fungal isolates have to be taken into account for assessing microbial control strategies.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► Subculturing of the entomopathogenic fungal isolate affects the tolerance of quiescent conidia (germination, ability to form colonies, and pathogenic activity) to wetting–drying stress. ► Water soluble extracts of leaf surface do not induce germination of quiescent conidia. ► Dry conditions on inert surfaces and on plant leaves are less favorable for conidial persistence than wet conditions.