| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4507442 | Crop Protection | 2009 | 5 Pages |
The geographical variability in Helicoverpa armigera susceptibility levels to Cry2Ab toxin expressed by transgenic maize (not currently commercialized in India) was determined through log dose probit assays conducted on populations collected during 2004–05 (from 25 districts) and 2007–08 (from 22 districts) across India. While 2004–05 is the year prior to commercialization of dual gene Bollgard-II-Bt cotton that expresses Cry2Ab in addition to Cry1Ac, 2007–08 is two years after its commercial release. LC50 values ranged from 6.0 to 28.6 μg Cry2Ab/ml of diet in 2004–05 and from 2.46 to 34.7 μg Cry2Ab/ml of diet in 2007–08. The IC50 range in 2004–05 and 2007–08 was from 0.31 to 2.3 μg/ml and 0.10–3.4 μg/ml of diet, respectively. The probit analysis data of 2004–05 can be used as baseline indices to monitor for changes in the H. armigera susceptibility to Cry2Ab, subsequent to the introduction of Bollgard-II cotton in India. The probit analysis data of 2007–08 showed that the baseline has not undergone any significant changes two years after cultivation of Bollgard-II in India. The relatively low toxicity of Cry2Ab and its higher expression late in the cotton season compared with Cry2Ab, compounded with its presence in Bt hybrids on a separate linkage group from Cry1Ac suggest problems of resistance in the future.
