Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4510126 | Field Crops Research | 2014 | 10 Pages |
•The leaf Nc curve developed in this paper has not been reported in previous research.•The present leaf Nc curve was lower than the reference plant Nc curve.•There was a significant positive relationship between NNI and N applications.•The leaf Nc curve can be used to diagnose the N status of winter wheat in eastern China.
Accurate measurement of the nitrogen (N) required for plant growth helps optimize grain yield, farm profits, and N-use efficiency. Critical nitrogen (Nc) curves have been developed to describe N dilution in plant tissues during crop growth and to estimate the N status of whole plants; however, Nc curves for leaves have yet to be constructed. We constructed and validated a leaf Nc curve for winter wheat based on leaf dry matter (LDM) and compared it with published whole-plant Nc curves to explore the potential for estimating leaf N status of winter wheat in eastern China. Four field experiments were conducted using a range of N fertilization levels (0–375 kg ha−1) applied to six wheat cultivars in eastern China. For our growth analyses, we determined LDM and leaf N concentration (LNC) in the developmental phases from spring regrowth to heading. The leaf Nc curve fit the following relationship: Nc = 3.05LDM−0.15 when LDM ranged from 0.52 to 2.64 t ha−1. However, when LDM was <0.52 t ha−1, we applied a constant leaf critical value of 3.37%. The curve we constructed was lower than the reference whole-plant Nc curve. The N nutrition index (NNI) ranged from 0.34 to 1.31 during the vegetative stage across the 2009–2011 seasons. There was a significant positive relationship between the difference values of NNI (ΔNNI) and N (ΔN) applications over the four wheat developmental stages. We obtained a root-mean-squared-error (RMSE) of 24.53 kg ha−1 between the predicted and observed ΔN values when testing the models with independent data. The leaf Nc dilution curve correctly identified N-limiting and non-N-limiting statuses and may be used as a reliable indicator of N stress during the growing season of winter wheat in eastern China.