Article ID Journal Published Year Pages File Type
4511562 Field Crops Research 2008 7 Pages PDF
Abstract
Two alternative PCR-based marker systems were tested for polymorphism in a set of fragrant and non-fragrant rice (Oryza sativa L.) varieties. Markers based on InDels (sequences with an insertion/deletion) gave reliable polymorphisms that split Basmatis and other varieties into different groups, however, markers based on Rim2/Hipa transposons were less reliable or informative. Of 42 InDels tested, 71% showed polymorphisms between Basmati varieties and non-Basmati indicas. A sub-set of nine InDel markers was selected as a reliable test to distinguish between Basmatis and other fragrant rice varieties using high-resolution agarose gels. Both marker systems tested are suitable for rice breeding and genetic analysis in laboratories where polyacrylamide gels or sequencers are not available.
Related Topics
Life Sciences Agricultural and Biological Sciences Agronomy and Crop Science
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